Abstract
Background and rationales: There are not much researches on the effects of heat exposure, specifically on oxidative DNA damage, among bakery employees. Physical labor-intensive baking exposes bakers to high temperatures, especially in traditional bakeries with uncovered ovens. An electronic heat measuring device index and serum 8-hydroxy-2-deoxy guanosine(8-OHdG) will be utilized in this study to quantify oxidative DNA damage and heat stress, respectively. The impact of heat exposure on oxidative DNA damage in the Duhok populace has not yet been studied in the literature. This study aimed to measure the impact of heat exposure on serum levels of oxidative DNA damage in bakers who work in high-temperature bakeries. Method: A case control study was carried out among 141 participants, 62 individuals exposed to heat (bakery workers) and 79 individuals unexposed to heat exposure as control group. 8-hydroxy 2-deoxy guanosine (8-OHdG) has been analyzed using Enzyme-Linked Immunosorbent Assay (ELISA). Results: The mean ±SD of 8-OHdG in bakery workers (13.78 ±5.89 ng/ml) were higher than those in healthy control individuals (1.55 ±0.75 ng/ml) with a statistically significantly differences (p<0.0001). Conclusion: High mean levels of 8-OHdG was found in exposed individuals (bakery workers) in comparison with control individuals unexposed to heat, suggesting that high-heat exposures have the risk of causing genetic effects and DNA damage.
References
Dong, X.S., West, G.H., Holloway-Beth, A., Wang, X., Sokas, R.K. (2019). Heat-related deaths among construction workers in the United States. American journal of industrial medicine, 62(12), pp.1047-1057.
Parsons, K. (2014). Human thermal environments: The effects of hot, moderate, and cold environments on human health, comfort, and performance. CRC Press. E book. doi.org/10.1201/b16750.
Al-Horani, R.A. (2022). A narrative review of exercise-induced oxidative stress: oxidative DNA damage underlined. The Open Sports Sciences Journal, 15(1), 1-12.
Argaud, L., Ferry, T., Le, Q.H., Marfisi, A., Ciorba, D., Achache, P., Ducluzeau, R., Robert, D. (2007). Short-and long-term outcomes of heatstroke following the 2003 heat wave in Lyon, France. Archives of Internal Medicine, 167(20), 2177-2183.
Li, L., Tan, H., Yang, H., Li, F., He, X., Gu, Z., Zhao, M., Su, L. (2017). Reactive oxygen species mediate heat stress-induced apoptosis via ERK dephosphorylation and Bcl-2 ubiquitination in human umbilical vein endothelial cells. Oncotarget, 8(8), 12902.
Starkov, A.A. (2008). The role of mitochondria in reactive oxygen species metabolism and signaling. Annals of the New York Academy of Sciences, 1147(1), 37-52.
Ray, P.D., Huang, B.W., Tsuji, Y. (2012). Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cellular Signalling, 24(5), 981-990.
Kjellstrom, T., Kovats, R.S., Lloyd, S.J., Holt, T. and Tol, R.S., (2009). The direct impact of climate change on regional labor productivity. Archives of Environmental & Occupational Health, 64(4), pp.217-227.
Han, J., Xu, X., Qin, H., Liu, A., Fan, Z., Kang, L., Fu, J., Liu, J., Ye, Q., (2013). The molecular mechanism and potential role of heat shock-induced p53 protein accumulation. Molecular and cellular Biochemistry, 378(1), 161-169.
Mahmoud, H.M., Altimimi, D.J. (2019). Evaluation of DNA Damage in Type 1 Diabetes Patients. Journal of Clinical & Diagnostic Research, 13(5), 4-5.
Pachauri, R.K., Reisinger, A. (2008). Climate change 2007. Synthesis report. Contribution of Working Groups I, II and III to the fourth assessment report.
Krishnamurthy, M., Ramalingam, P., Perumal, K., Kamalakannan, L.P., Chinnadurai, J., Shanmugam, R., Srinivasan, K. and Venugopal, V., 2017. Occupational heat stress impacts on health and productivity in a steel industry in Southern India. Safety and Health at Work, 8(1), 99-104.
Rooney, C., McMichael, A.J., Kovats, R.S., Coleman, M.P. (1998). Excess mortality in England and Wales, and in Greater London, during the 1995 heatwave. Journal of Epidemiology & Community Health, 52(8), 482-486.
Sawka, M.N., Latzka, W.A., Montain, S.J., Cadarette, B.S., Kolka, M.A., Kraning, K.K., Gonzalez, R.R. (2001). Physiologic tolerance to uncompensable heat: intermittent exercise, field vs laboratory. Medicine and Science in Sports and Exercise, 33(3), 422-430.
Aragon-Vargas, L.F., Moncada-Jimenez, J., Hernandez-Elizondo, J., Barrenechea, A., Monge-Alvarado, M. (2009). Evaluation of pre-game hydration status, heat stress, and fluid balance during professional soccer competition in the heat. European Journal of Sport Science, 9(5), 269-276.
Lucas, R.A., Bodin, T., García-Trabanino, R., Wesseling, C., Glaser, J., Weiss, I., Jarquin, E., Jakobsson, K., Wegman, D.H. (2015). Heat stress and workload associated with sugarcane cutting-an excessively strenuous occupation. Extrem Physiol Med., 4(1), A23.
Kantidze, O.L., Velichko, A.K., Luzhin, A.V., Razin, S.V. (2016). Heat stress-induced DNA damage. Acta Naturae, 8(2), 75-78.
Goukassian, D., Gad, F., Yaar, M., Eller, M.S., Nehal, U.S., Gilchrest, B.A. (2000). Mechanisms and implications of the age-associated decrease in DNA repair capacity. The FASEB Journal, 14(10), 1325-1334.
Hameed, E.A., El Wakkad, A., Hassan, N.E.M., Sherif, L., Abd El-Shaheed, A., Sebii, H., El Zayat, S. (2012). 434 Relationship between Obesity and 8-Hydroxy 2-Deoxy Guanosine as an Oxidative Marker in Obese Adolescents of Giza. Archives of Disease in Childhood, 97(Suppl 2), A127-A128.
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