Effect of Four Plant Extracts on Opportunistic Bacteria: Sphingomonas paucimobilis and Enterococcus faecium


Otitis media
Sphingomonas Paucimobilis
Enterococcus Faecium
Brassicaceae family
plant extracts

How to Cite

Mohammad, G. A., & Al-Wattar, M. (2023). Effect of Four Plant Extracts on Opportunistic Bacteria: Sphingomonas paucimobilis and Enterococcus faecium. Journal of Life and Bio Sciences Research , 4(02), 58 - 62. https://doi.org/10.38094/jlbsr402106


The emergence of new bacterial species in different infections, such as Sphingomonas paucimobilis and Enterococcus faecium needs materials to get rid of, especially if these materials are of 100% natural origin, however, repeated experiments must be conducted from different materials for the same bacterial species until reaching the target material. The research has investigated about if two nosocomial infective bacteria Sphingomonas paucimobilis and Enterococcus faecium could influence with some plants (Lepidium sativum, Sinapis arvensis, Eruca sativa and Raphanus sativus) belong to the Brassicaceae family which are mainly known containing compounds that are effective in combating pathogenic bacteria, using the disk diffusion method. The result was no inhibition zone around the disks saturated with water and alcoholic extracts separately by all plants against these two bacterial species tested. The authors concluded that these bacteria might have adaptation from previous exposure to their environment. From our result, it is clear that there is a need to test extracts from other plants to resist these two bacterial species, which may pose a health risk, especially since they are previously registered to be resistant to antibiotics.



Adam, S.I., Salih, S. A., Abdelgadir, W.S. (2011). "In vitro" Antimicrobial assessment of "Lepidium sativum" L. seeds extracts. Asian Journal of Medical Sciences, 3(6), 261-266.

Ahmad, F., Hasan, I., Chishti, D.K., Ahmad, H. (2012). Antibacterial activity of Raphanus sativus Linn. seed extract. Global Journal of Medical Research, 12(11), 25-34.

Ahmad, N. H., Mohammad, G. A. (2019). Evaluation of Some Material to inhibit Biofilm Formed by Acinetobacter baumannii Isolates. Tikrit Journal of Pure Science, 24(4), 19-24.?

Akrayi, H.F., Tawfeeq, J.D. (2012). Antibacterial activity of Lepidium sativum and Allium porrum extracts and juices against some gram positive and gram negative bacteria. Medical Journal of Islamic World Academy of Sciences, 20(1), 10-16.

Al-Hasan, M.N., Eckel-Passow, J.E., Baddour, L.M. (2011). Influence of referral bias on the clinical characteristics of patients with Gram-negative bloodstream infection. Epidemiology & Infection, 139(11), 1750-1756.

Al-Qudah, M.A., Al-Jaber, H.I., Muhaidat, R., Hussein, E.I., Abdel, A.A., Hamid, A.S. (2011). Chemical composition and antimicrobial activity of the essential oil from Sinapis alba L. and Sinapis arvensis L. (Brassicaceae) growing wild in Jordan. Res. J. Pharm. Biol. Chem. Sci, 2(4), 1136-1144.

Arias, C.A., Murray, B.E. (2012). The rise of the Enterococcus: beyond vancomycin resistance. Nature Reviews Microbiology, 10(4), 266-278.

Ashebir, M., Ashenafi, M. (1999). Assessment of the antibacterial activity of some traditional medicinal plants on some food-borne pathogens. Ethiopian Journal of Health Development, 13(3).

Besufekad, Y., Beri, S., Adugnaw, T., Beyene, K. (2018). Antibacterial activity of Ethiopian Lepidium sativum L. against pathogenic bacteria. Journal of Medicinal Plants Research, 12(6), 64-68.

Byappanahalli, M.N., Nevers, M.B., Korajkic, A., Staley, Z. R., Harwood, V.J. (2012). Enterococci in the environment. Microbiology and Molecular Biology Reviews, 76(4), 685-706.

Camacho, C., Arias-Palacios, J., Rodríguez, A. (2019). Assessment of the antibacterial capacity of extracts of Sinapis alba L. by the method of plates and wells. Pharmacology Online, 2, 329-335.

de la Maza, L.M, Pezzlo M.T., Bittencourt C.E., and Peterson E.M. (2020). Color atlas of medical bacteriology, Third Edition ASM Press, Washington, Wiley.

Gulfraz, M., Sadiq, A., Tariq, H., Imran, M., Qureshi, R., Zeenat, A. (2011). Phytochemical analysis and antibacterial activity of Eruca sativa seed. Pak. J. Bot, 43(2), 1351-1359.

Guzman Prieto, A.M., van Schaik, W., Rogers, M.R., Coque, T.M., Baquero, F., Corander, J., Willems, R.J. (2016). Global emergence and dissemination of enterococci as nosocomial pathogens: attack of the clones? Frontiers in microbiology, 7, 788.

Ionescu, M.I., Neagoe, D.?., Cr?ciun, A.M., Moldovan, O.T. (2022). The Gram-negative bacilli isolated from caves—Sphingomonas paucimobilis and Hafnia alvei and a review of their involvement in human infections. International Journal of Environmental Research and Public Health, 19(4), 2324.

Jardine, J.L., Abia, A.L.K., Mavumengwana, V., Ubomba-Jaswa, E. (2017). Phylogenetic analysis and antimicrobial profiles of cultured emerging opportunistic pathogens (phyla Actinobacteria and Proteobacteria) identified in hot springs. International Journal of Environmental Research and Public Health, 14(9), 1070.

Kawahara, K., Kuraishi, H., Zähringer, U. (1999). Chemical structure and function of glycosphingolipids of Sphingomonas spp and their distribution among members of the ?-4 subclass of Proteobacteria. Journal of Industrial Microbiology and Biotechnology, 23(4-5), 408-413.

Khatib, R., Al-Makky, K. (2021). Anti-oxidant and anti-bacterial activities of Sinapis alba l.(leaves, flowers and fruits) grown in Syria. Bulletin of Pharmaceutical Sciences. Assiut, 44(2), 339-346.

Kirby, W.M. and Bauer, A.M. (1966). Antibiotic susceptibility testing by a standardized single disc method. J. Clin. Pathol, 45, 493-496.

Laupland, K.B., Paterson, D.L., Stewart, A.G., Edwards, F., Harris, P.N. (2022). Sphingomonas paucimobilis bloodstream infection is a predominantly community-onset disease with significant lethality. International Journal of Infectious Diseases, 119, 172-177.

Monticelli, J., Knezevich, A., Luzzati, R., Di Bella, S. (2018). Clinical management of non-faecium non-faecalis vancomycin-resistant enterococci infection. Focus on Enterococcus gallinarum and Enterococcus casseliflavus/flavescens. Journal of Infection and Chemotherapy, 24(4), 237-246.

Nishimura, K., Ikarashi, M., Yasuda, Y., Sato, M., Cano Guerrero, M., Galipon, J., Arakawa, K. (2021). Complete genome sequence of Sphingomonas paucimobilis strain Kira, isolated from human neuroblastoma SH-SY5Y cell cultures supplemented with retinoic acid. Microbiology Resource Announcements, 10(6), e01156-20.

Nowicki, D., Krause, K., Szamborska, P., ?ukowska, A., Cech, G.M., Szalewska-Pa?asz, A. (2021). Induction of the stringent response underlies the antimicrobial action of aliphatic isothiocyanates. Frontiers in Microbiology, 11, 591802.

?Parnell, J., Curtis, T. (2012). Webb's An Irish Flora. Cork University Press., ISBN 978-185918-4783 .

Quirante-Moya, S., García-Ibañez, P., Quirante-Moya, F., Villaño, D., Moreno, D.A. (2020) The role of brassica bioactives on human health: are we studying it the right way? Molecules. 25.

Selah, M.T., Mohammad, G.A. (2021). Ability of three species of enterobacter bacteria to synthesize iron nanoparticles and detection of the efficacy to inhibitory effect on other pathogenic bacteria. Biochemical and Cellular Archives, 21, 2085-2090.?

Sikorska-Zimny, K., Beneduce, L. (2021). The glucosinolates and their bioactive derivatives in Brassica: a review on classification, biosynthesis and content in plant tissues, fate during and after processing, effect on the human organism and interaction with the gut microbiota. Critical Reviews in Food Science and Nutrition, 61(15), 2544-2571.

Ugolini, L., Scarafile, D., Matteo, R., Pagnotta, E., Malaguti, L., Lazzeri, L., Braschi, I. (2021). Effect of bioactive compounds released from Brassicaceae defatted seed meals on bacterial load in pig manure. Environmental Science and Pollution Research, 28, 62353-62367

Zhen, X., Lundborg, C.S., Sun, X., Hu, X., Dong, H. (2019). Economic burden of antibiotic resistance in ESKAPE organisms: a systematic review. Antimicrobial Resistance & Infection Control, 8, 1-23.

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