Determination of IgG and IgM against COVID- 19 for Recovered Patients at Different Intervals and by Different Techniques


Rapid test cassette

How to Cite

Al- Hadidi, E., & Al-Tobje, M. (2022). Determination of IgG and IgM against COVID- 19 for Recovered Patients at Different Intervals and by Different Techniques. Journal of Life and Bio Sciences Research , 3(01), 07 - 11.


The research aimed to determine the concentrations of IgG and IgM antibodies for different intervals after recovery from Covid-19, namely 4, 6 and 8 months; the research also aimed to identify the accordance between two types of immunity techniques used to identify the existence of antibodies. The results showed the existence of IgG antibodies with a percentage of (94.03%), and IgM antibodies with a percentage of 55.22% in individuals under study when using ELISA technique. The percentages of these antibodies were (86.56%) for IgG and (16.42%) for IgM when using Rapid test cassette in diagnosis; the matching was 67% between the two methods. Samples of the control group also showed the presence of IgG and IgM with percentages of 68% and 88% respectively.The average concentrations of IgG antibodies were 33.05, 43.21 and 37.53 after 4, 6 and 8 months after infection respectively; the peak was at the 6th month after infection. The averages of IgM were 14.45, 18.52 and 19.18 after 4, 6 and 8 months of being infected respectively; the peak was at the 8th month after infection.


Bastos, L., Tavaziva, M., Abidi, G., Campbell, S.K., Haraoui, J.R., Johnston, L.P., Lan, J.C., et al. (2020). Diagnostic Accuracy Of Serological Tests For COVID-19: Systematic Review And Meta-Analysis. BMJ, 1,370,M2516.

Beaudoin-Bussières, G., Laumaea, A., Anand, S.P., Prévost, J., Gasser, R., Goyette, G., Medjahed, H., et al. (2020). Decline of Humoral Responses against SARS-CoV-2 Spike in Convalescent Individuals. mBio, 11(5), e02590-20.

Brochot, E., Demey, B., Touzé, A., Belouzard, S., Dubuisson, J., Schmit, J.L., Duverlie, G., et al. (2020). Anti-spike, Anti-nucleocapsid and Neutralizing Antibodies in SARS-CoV-2 Inpatients and Asymptomatic Individuals. Front Microbiol, 11, 584251.

Cervia, C., Nilsson, J., Zurbuchen, Y., Valaperti, A., Schreiner, J., Wolfensberger, A., Raeber, M.E., et al. (2021). Systemic and mucosal antibody responses specific to SARS-CoV-2 during mild versus severe COVID-19. J Allergy Clin Immunol, 147(2),545-557.e9.

Choe, P.G., Kim, K.H., Kang, C.K., Suh, H.J., Kang, E., Lee, S.Y., et al. (2021). Antibody Responses 8 Months after Asymptomatic or Mild SARS-CoV-2 Infection. Emerg Infect Dis, 27(3),928-931.

Crawford, K.H.D., Dingens, A.S., Eguia, R., Wolf, C.R., Wilcox, N., Logue, J.K., Shuey, K., et al. (2021). Dynamics of Neutralizing Antibody Titers in the Months After Severe Acute Respiratory Syndrome Coronavirus 2 Infection. J Infect Dis, 223 (2),197-205.

Cui, J., Li, F., Shi, Z.L. (2019). Origin and evolution of pathogenic corona-viruses. Nat Rev Microbial, 17,181-192.

Dan, J.M., Mateus, J., Kato, Y., Hastie, K.M., Yu, E.D., Faliti, C.E., Grifoni, A., et al. (2021). Immunological memory to SARS-CoV-2 assessed for up to 8 months after infection. Science, 371(6529), eabf4063.

Dave, M., Poswal, L., Bedi, V., Regar, L., Vijayvargiya, R., Sharma, M. Deval, N. (2020). Study of Antibody-based Rapid Card Test in COVID-19 Patients Admitted in a Tertiary Care COVID Hospital in Southern Rajasthan. Journal Indian Academy of Clinical Medicine. 21, 7-11.

Deeks, J.J., Dinnes, J., Takwoingi, Y., Davenport, C., Spijker, R., Taylor-Phillips, S., Adriano, A. (2020). Cochrane COVID-19 Diagnostic Test Accuracy Group. Antibody Tests for Identification of Current and Past Infection with SARS-CoV-2. Cochrane Database Syst Rev. 6 (6), CD013652.

Figueiredo-Campos, P., Blankenhaus, B., Mota, C., Gomes, A., Serrano, M., Ariotti, S., Costa, et al. (2020). Seroprevalence of Anti-SARS-CoV-2 Antibodies in COVID-19 Patients and Healthy Volunteers up to 6 Months Post Disease Onset. Eur J Immunol. 50(12),2025-2040.

Gluck, V., Grobecker, S., Tydykov, L., Salzberger, B., Glück, T., Weidlich, T., Bertok, M., et al. (2021). SARS-CoV-2-Directed Antibodies Persist for More Than Six Months in a Cohort with Mild to Moderate COVID-19. Infection. 49(4),739-746.

Goudouris, E.S. (2021). Laboratory Diagnosis of COVID-19. J. Pediatr. Rio J, 97, 7–12.

Grandjean, L., Saso, A., Torres, A., Lam, T., Hatcher, J., Thistlethwayte, R., Harris, M. (2020). Humoral Response Dynamics Following Infection with SARSCoV-2. medRxiv, 2020.07.16.20155663.

Gudbjartsson, D.F., Norddahl, G.L,, Melsted P, Gunnarsdottir K, Holm H, Eythorsson E, Arnthorsson AO, et al. (2020). Humoral Immune Response to SARS-CoV-2 in Iceland. N Engl J Med, 383(18),1724-1734.

Ibarrondo, F.J., Fulcher, J.A., Goodman-Meza, D., Elliott, J., Hofmann, C., Hausner, M.A., Ferbas, K.G., et al. Rapid Decay of Anti-SARS-CoV-2 Antibodies in Persons with Mild COVID-19. N Engl J Med, 383(11),1085-1087.

Isho, B., Abe, K.T., Zuo, M., Jamal, A.J., Rathod, B., Wang, J.H., Li, Z., et al. (2020). Persistence of serum and saliva antibody responses to SARS-CoV-2 spike antigens in COVID-19 patients. Sci Immunol, 8,5(52), eabe5511.

Iyer, A.S., Jones, F.K., Nodoushani, A., Kelly, M., Becker, M., Slater, D, Mills, R., et al. (2020). Persistence and decay of human antibody responses to the receptor binding domain of SARS-CoV-2 spike protein in COVID-19 patients. Sci Immunol, 5, eabe0367.

Kirkwood, B.R., Sterne, J.A.C. (1988). Medical Statstic. 6th ed. Blackwell publication company.

Kontou, P.I., Braliou, G.G., Dimou, N.L., Nikolopoulos, G., Bagos, P.G. (2020). Antibody Tests in Detecting SARS-CoV-2 Infection: A Meta-Analysis. Diagnostics (Basel). 19,10(5),319.

Krajewski, R., Golebiowska, J., Makuch, S., Mazur, G., Agrawal, S. (2020). Update on serologic testing in COVID-19. Clin Chim Acta, 510,746-750.

Lassauniere, R., Frische, A., Harboe, Z.B., Nielsen, A.C.Y., Fomsgaard, A., Krogfelt, K.A., Jørgensen, C.S. (2020). Evaluation of nine commercial SARS-CoV-2 immunoassays. Medrxiv, 1-15.

Lee, C.Y., Lin, R.T.P., Renia, L., Ng, L.F.P. (2020). Serological Approaches for COVID-19: Epidemiologic Perspective on Surveillance and Control. Front Immunol, 24,11,879.

Lee, W.T., Girardin, R.C., Dupuis, A.P., Kulas, K.E., Payne, A.F., Wong, S.J., Arinsburg, S., et al. (2020). Neutralizing Antibody Responses in COVID-19 Convalescent Sera. J Infect Dis, 4,223(1),47-55.

Li, C., Zhao, C., Bao, J., Tang, B., Wang, Y., Gu, B. (2020). Laboratory diagnosis of coronavirus disease-2019 (COVID-19). Clin Chim Acta, 510,35-46.

Li, Z., Yi, Y., Luo, X., Xiong, N., Liu, Y., Li, S., Sun, R., et al. (2020). Development and clinical application of a rapid IgM-IgG combined antibody test for SARS-CoV-2 infection diagnosis. J Med Virol, 92(9),1518-1524.

Liu, C., Yu, X., Gao, C., Zhang, L., Zhai, H., Hu, Y., Liu, E., et al. (2021). Characterization of antibody responses to SARS-CoV-2 in convalescent COVID-19 patients. J Med Virol, 93(4),2227-2233.

Liu, X., Wang, J., Xu, X., Liao, G., Chen, Y., Hu, C.H. (2020). Patterns of IgG and IgM antibody response in COVID-19 patients. Emerg Microbes Infect, 9(1),1269-1274.

Long, Q.X., Liu, B.Z., Deng, H.J., Wu, G.C., Deng, K., Chen, Y.K., Liao, P., et al. (2020). Antibody responses to SARS-CoV-2 in patients with COVID-19. Nat Med, 26(6),845-848.

Lou, B., Li, T.D., Zheng, S.F., Su, Y.Y., Li, Z.Y., Liu, W., Yu, F., et al. (2020). Serology characteristics of SARS-CoV-2 infection after exposure and post-symptom onset. Eur Respir J, 27,56(2),2000763.

Mandrekar, J.N. (2010). Simple Statistical Measures for Diagnostic Accuracy Assessment. J Thorac Oncol, 5, 763–764.

Marklund, E., Leach, S., Axelsson, H., Nyström, K., Norder, H., Bemark, M., Angeletti, D., et al. (2020). Serum-IgG responses to SARS-CoV-2 after mild and severe COVID-19 infection and analysis of IgG non-responders. PLoS One, 21,15(10),e0241104. Erratum in: PLoS One, 2021,16(10),e0258401.

McHugh, M.L. Interrater Reliability: The Kappa Statistic. (2012). Biochem Medica, 22(3),276–82.

Pan, Y., Li, X., Yang, G., Fan, J., Tang, Y., Zhao, J., Long, X., et al. (2020). Serological Immunochromatographic Approach in Diagnosis with SARS-CoV-2 Infected COVID-19 Patients. J Infect, 81(1),e28-e32.

Rastawicki, W., Rokosz-Chudziak, N. (2020). Characteristics and Assessment of the Usefulness of Serological Tests in the Diagnostic of Infections Caused by Coronavirus SARS-CoV-2 on the Basis of Available Manufacturer's Data and Literature Review. Przegl Epidemiol, 74(1),49-68.

Ravi, N., Cortade, D.L., Ng, E., Wang, S.X. (2020). Diagnostics for SARS-CoV-2 Detection: A Comprehensive Review of the FDA-EUA COVID-19 Testing Landscape. Biosens Bioelectron, 165,112454.

Röltgen, K., Powell, A.E., Wirz, O.F., Stevens, B.A., Hogan, C.A., Najeeb, J., Hunter, M., et al. (2020). Defining the Features and Duration of Antibody Responses to SARS-CoV-2 Infection Associated with Disease Severity and Outcome. Sci Immunol, 5(54),eabe0240.

Sasisekharan, V., Pentakota, N., Jayaraman, A., Tharakaraman, K., Wogan, G.N., Narayanasami, U. (2021). Orthogonal Immunoassays for IgG Antibodies to SARS-CoV-2 Antigens Reveal that Immune Response Lasts Beyond 4 Mo Post Illness Onset. Proc Natl Acad Sci U S A. 118(5),e2021615118.

Self, W.H., Tenforde, M.W., Stubblefield, W.B., Feldstein, L.R., Steingrub, J.S., Shapiro, N.I., Ginde A.A., et al. (2020). CDC COVID-19 Response Team; IVY Network. Decline in SARS-CoV-2 Antibodies After Mild Infection Among Frontline Health Care Personnel in a Multistate Hospital Network - 12 States. MMWR Morb Mortal Wkly Rep, 69(47), 1762-1766.

Seow, J., Graham, C., Merrick, B., Acors, S., Pickering, S., Steel, K.J.A., Hemmings, O. et al. (2020). Longitudinal Observation and Decline of Neutralizing Antibody Responses in the Three Months Following SARS-Cov-2 Infection In Humans. Nature Microbiology, 5,1598-607.

Shu, H., Wang, S., Ruan, S., Wang, Y., Zhang, J., Yuan, Y., Liu, H., et al. (2020). Dynamic Changes of Antibodies to SARS-CoV-2 in COVID-19 Patients at Early Stage of Outbreak. Virol Sin, 35(6),744-751.

Tan, Y., Liu, F., Xu, X., Ling, Y., Huang, W., Zhu, Z., Guo, M., et al. (2020). Durability of Neutralizing Antibodies and T-cell Response Post SARS-CoV-2 Infection. Front Med, 14(6),746-751.

To, K.K., Tsang, O.T., Leung, W.S., Tam, A.R., Wu, T.C., Lung, D.C., Yip, C.C., et al. (2020). Temporal Profiles Of Viral Load In Posterior Oropharyngeal Saliva Samples and Serum Antibody Responses During Infection by SARS-Cov-2: An Observational Cohort Study. Lancet Infect Dis, (5),565-574.

Vengesai, A., Midzi, H., Kasambala, M., Mutandadzi, H., Mduluza-Jokonya, T.L., Rusakaniko, S., Mutapi, F., et al. (2021). A Systematic and Meta-Analysis Review on The Diagnostic Accuracy of Antibodies in The Serological Diagnosis Of COVID-19. Syst Rev, 10(1),155.

Wajnberg, A., Amanat, F., Firpo A., Altman, D.R. Bailey, M.J., Mansour, M., McMahon, M., et al. (2020). SARS-CoV-2 Infection Induces Robust, Neutralizing Antibody Responses That are Stable for at Least Three Months. medRxiv, 2020.07.14.20151126.

Wang, H., Ai, J., Loeffelholz, M.J., Tang, Y.W., Zhang, W. (2020). Meta-Analysis of Diagnostic Performance of Serology Tests for COVID-19: Impact of Assay Design and Post-Symptom-Onset Intervals. Emerg Microbes Infect, 9(1),2200-2211.

Wang, K., Long, Q.X., Deng, H.J., Hu, J., Gao, Q.Z., Zhang, G.J., He, C.L., et al. (2021). Longitudinal Dynamics of the Neutralizing Antibody Response to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection. Clin Infect Dis. 73(3),e531-e539.

Wang, P., Liu, L., Nair, M.S., Yin, M.T., Luo, Y., Wang, Q., Yuan, T., et al. (2020). SARS-CoV-2 Neutralizing Antibody Responses are More Robust in Patients with Severe Disease. Emerg Microbes Infect, 9(1),2091-2093.

WHO. Laboratory testing for coronavirus disease 2019 (COVID-19) in suspected human cases: interim guidance. (2020). World Health Organization; 2020.

Wölfel, R., Corman, V.M., Guggemos, W., Seilmaier, M., Zange, S., Müller, M.A., Niemeyer, D., et al. (2020). Virological assessment of hospitalized patients with COVID-2019. Nature. 581(7809),465-469. Erratum in: Nature, 588(7839), E35.

Wolff, F., Dahma, H., Duterme, C., Van den Wijngaert, S., Vandenberg, O., Cotton, F., Montesinos, I. (2020). Monitoring Antibody Response Following SARS-CoV-2 Infection: Diagnostic Efficiency of 4 Automated Immunoassays. Diagn Microbiol Infect Dis, 98(3),115140.

Xu, Y., Xiao, M., Liu, X., Xu, S., Du, T., Xu, J., Yang, Q., et al. (2020). Significance of Serology Testing to Assist Timely Diagnosis of SARS-CoV-2 Infections: Implication from a Family Cluster. Emerg Microbes Infect, 9(1),924-927.

Yang, T., Gentile, M., Shen, C.F., Cheng, C.M. (2020). Combining Point-of-Care Diagnostics and Internet of Medical Things (IoMT) to Combat the COVID-19 Pandemic. Diagnostics (Basel), 10(4),224.

Zhao, J., Yuan, Q., Wang, H., Liu, W., Liao, X., Su, Y., Wang, X., et al. (2020). Antibody Responses to SARS-CoV-2 in Patients with Novel Coronavirus Disease 2019. Clin Infect Dis, 71(16),2027-2034.

Zhong, L., Chuan, J., Gong, B., Shuai, P., Zhou, Y., Zhang, Y., Jiang, Z. (2020). Detection of serum IgM and IgG for COVID-19 diagnosis. Sci. China Life Sci, 63,777.

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