Mutagenic testing of portable water is fast becoming an important protocol for assessing water quality. Two potable water samples were selected from both ground and surface water sources within the Okerenkoko community, Gbaramatu Kingdom, Delta State Nigeria. Ame’s test was employed in the assessment of potential carcinogens in the potable water available to the community. The study was carried out in triplicate to broaden the reproducibility of the study. Sensitivity of the test organism was evaluated using the Salmonella TA98 and Salmonella TA100 which was reconstituted for the study. The average revertant colonies from the study was enumerated to indicate that the test conducted on S3OK (Surface water) had 428.1 and 637.84 revertant colonies using Salmonella TA 100 for both +S9 mix and -S9 mix-conditions respectively while similar replica result was for Salmonella TA 98 was 629.7 and 66.12 revertant colonies. The samples obtained from S1OK had slightly lower number of revertant colonies of 209.16 and 115.94 colonies for Salmonella T.A 100 while for Salmonella T.A 98 had 116.82 and 108.90 colonies for treatments containing S9 mix preparations. The study identified the test organism was Salmonella T.A 98 was more sensitive to the carcinogen present in the S3OK sample being used by a wide population of indigenes of the lower economic class. This study further buttresses the need to develop a more robust monitoring protocol for packaged water sources in Nigeria. Regulatory agencies such as NAFDAC must enforce strict intervention limits for potable water sources.
Agbalagba, O.E., Agbalagba, O.H., Ononugbo, C.P., Alao, A.A. (2011). Investigation into the physico-chemical properties and hydrochemical processes of groundwater from commercial boreholes In Yenagoa, Bayelsa State, Nigeria. African Journal of Environmental Science and Technology, 5(7), 473-481.
Agwa, O.K., Eze, N.J., Okpokwasili, G.C. (2017). Mutagenic Potentials of Potable Water from Ground Sources. The Open Biotechnology Journal, 11(1). 81-88.
Ames, B. N. (1971). The detection of chemical mutagens with enteric bacteria. Chemical Mutagens: Principles and Methods for Their Detection Volume 1, 267-282.
Atubi, A.O. (2011). Effects of warri refinery effluents on water quality from the Iffie River, Delta State, Nigeria. American Review of Political Economy, 9(1), 45-56.
Batista, N.J.C., Cavalcante, A.A.D.C.M., de Oliveira, M.G., Medeiros, E.C. N., Machado, J.L., Evangelista, S.R., da Silva, J. (2016). Genotoxic and mutagenic evaluation of water samples from a river under the influence of different anthropogenic activities. Chemosphere, 164, 134-141.
Bushini, A., Carboni, P., Frigerio, S., Furlini, M., Marabini, L., Monarca, S., Rossi, C. (2004). Genotoxicity and cytotoxicity assessment in lake drinking water produced in a treatment Plant. Mutagenesis, 19(5), 341-347.
EBPI, (2009). Muta-Chromoplate Bacterial Strain Kit Version 3.3 Instructions for Use pp. 1-16.
Guan, Y., Wang, X., Wong, M., Sun, G., An, T., Guo, J., Zhang, G. (2017). Evaluation of genotoxic and mutagenic activity of organic extracts from drinking water sources. PloS one, 12(1), e0170454.
Lv, J., Wang, Y. (2019). PMF receptor models and sequential Gaussian simulation to determine the quantitative sources and hazardous areas of potentially toxic elements in soils. Geoderma, 353, 347-358.
Ohe, T., White, P.A., DeMarini, D.M., (2003). Mutagenic characteristics of river waters flowing through large metropolitan areas in North America. Mutation Research, 534, 101-112.
Pan, E., Zhang, Q., Yang, F., Hu, W., Xu, Q., Liang, C., Wang, C. (2014). Study on of the current status of volatile organic compounds pollution in typical rural drinking water and the relationship between its concentration and health of the population, in Huai'an, Jiangsu. Zhonghua Liu Xing Bing Xue Za Zhi, 35(10), 1105-1108.
Sheshe, M.U., Magashi, A.M. (2014). Assessment of physicochemical quality of sachet water produced in selected local government areas of Kano Metropolis, Kano State-Nigeria. Bayero Journal of Pure and Applied Sciences, 7(2), 31-35.
Umbuzeiro, G.D.A., Roubicek, D.A., Sanchez, P. S., Sato, M.I.Z. (2001). The Salmonella mutagenicity assay in a surface water quality monitoring program based on a 20-year survey. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 491(1-2), 119-126.
Wang, A., Pu, K., Dong, B., Liu, Y., Zhang, L., Zhang, Z., Zhu, Y. (2013). Role of surface charge and oxidative stress in cytotoxicity and genotoxicity of graphene oxide towards human lung fibroblast cells. Journal of Applied Toxicology, 33(10), 1156-1164.
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