Toxicity of Soursop Leaf Powder and Its Relevance in Determining The Micronutrient Status in Formulated Complementary Food
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Keywords

Pigeon pea
Acha
Soursop leaf powde
Complementary food
Micronutrient
Bioassay

How to Cite

Mbaeyi-Nwaoha, I. E., Ani, E., & Okoronkwo, N. C. (2021). Toxicity of Soursop Leaf Powder and Its Relevance in Determining The Micronutrient Status in Formulated Complementary Food. Journal of Life and Bio Sciences Research , 2(01), 13 - 20. https://doi.org/10.38094/jlbsr20124

Abstract

Complementary food was produced from blends of hungry rice (A), pigeon pea (P) and soursop leaves (S). The raw materials were washed with portable water, dried at room temperature, milled with hammer mill, fermented for 24 hours at 28 ± 2 °C (28 ± 2 °C), oven-dried at 50 °C for 12 hours, remilled, sieved to 1 mm pore size and packaged in polyethylene bags for further analysis. The samples were in the ratio of 70:30:0 (sample APS), 65:30:5 (sample APS1), 60:30:10 (sample APS2) and 55:30:15 (sample APS3). Toxicity test for lethal dose (LD50) was carried out on the soursop leaves. Bioassay was carried out with male albino rats for 28 days including acclimatization period of 7 days. Feed intake and weight gain of experimental rats were recorded daily and weekly. Blood serum was collected before and after feeding trials for analyzing bioavailability of the selected micronutrients. The data were subjected to one-way analysis of variance. Means were separated using the Duncan’s multiple range test and significance was accepted at probability level of 0.05 %. The toxicity test (LD50) indicated safety of soursop leaf as an infusion (oral administration) at lower doses of 10-1000 mg/kg body weight of rats. The bioassay revealed that food intake was significantly (p < 0.05) different among the samples in the first, second and third week. Rats that ate normal rat chow had the highest food intake while the rats that ate APS3 had the lowest food intake. Weight gain was highest in rats that ate rat chow while it was lowest in the rat that ate APS3. Bioavailability of selected micronutrient revealed that calcium content had the highest bioavailability in rats fed with rat chow and lowest in AP. Sample of APS1 had the highest iron bioavailability (47.83 %) among the fortified samples and the rat chow. Zinc had the highest bioavailability (52.86 %) in APS1. The work revealed that selected vitamins were most available in APS2 and the selected minerals were most available in sample APS1.

https://doi.org/10.38094/jlbsr20124
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References

Arthur, F. K. N., Woode, E., Terlabi, E.O and Larbie, C. (2011). Evaluation of acute and subchronic toxicity of (Annona muricata Linn) aqueous extract in animals. European Journal of Experimental Biology, 1(4): 115 – 124.

Arueya, G. L and Osundahunsi, O. F. (2014). Effect of external extruded soybean-cocoa and cornstarch based complementary food on some histopathological parameters of rats. African Journal of Food Science 8(8): 419 – 426.

Badamosi, E. J., Ibrahim, L. M. and Temple, V. J. (1995). Nutritional evaluation of a locally formulated weaning food. West Africa Journal of Biological Sciences, 3: 85 - 93.

Echendu, C. A., Obizoba, I. C., Anyika, J. U.and Ojimelukwe, P. C. (2009). Changes in chemical composition of treated and untreated hungry rice acha (Digitaria exilis). Pakistan Journal of Nutrition, 8:1779 - 1785.

Eka, P. N. R., Tuti, S. S. and Retino, W. A. (2012). The breast of anti-cancer from leaf extract of Annona muricata against cell line in T47D. International Journal of Applied Science and Technology, 2(1): 157 – 165.

Ene-Obong, H. N. and Obizoba, I. C (1995). Protein quality of some Nigerian traditional diets based on the African yam bean (Sphenostylis sternocarpa) and pigeon pea (Cajanus cajan). Plant Food and Human Nutrition, 48: 297 - 309.

FAO (1997). Preventing micronutrient malnutrition: A guide to food-based approaches. A Manual for policy makers and programme planners. Food and Agricultural Organization of the United Nations and International Life Science Institute, Washington DC, pp. 2 - 6.

FAO (2000). Energy and protein requirement: Report of joint FAO/WHO Expert Consultation WHO Technical report series 724, WHO Geneva, Switzerland.

FAO / WHO (1998). Preparation and use of food-based dietary guidelines. A report of joint FAO/WHO consultation. WHO Technical Report Series 880, Geneva.

Fasoyiro, S. B., Akande, S. R., Arowora, K. A., Sodeko, O. O., Suilaiman, P. O., Olapade, C. O., and Odiri, E. C. (2010). Physio-chemical and sensory properties of pigeon pea (Cajanus cajan) flours. African Journal of Food Science, 4(3): 120 - 126.

Federal Ministry of Health (FMOH) (1999). Food-based dietary guideline for Nigeria. Nutrition Division, Abuja.

Fernandez, D. P., Vanderjagt, D. J., Williams, M., Huang, Y. S., Chuang, L., Millson, M., Andrews, R., Pastuszyn, A. and Glew, R. H. (2002). Fatty acid, amino acid and trace mineral analysis of five weaning foods, from Jos Nigeria. Plant Foods for Human Nutrition, 57: 257 - 274.

Guptill, K. S., Estrey, S. A., Oni, G. A. and Brown, K. H (1993). Evaluation of a face-to- face weaning food intervention in Kwara State, Nigeria: Knowledge, trial and adoption of a home prepared weaning food. Social Science Medicine 36: 665 – 672.

Ihekoronye, A. I. and Ngoddy, P. O. (1985). Integrated food science and technology for the tropics: Macmillan Publishers Ltd., Basingstoke, London. pp. 95 - 105.

Johnsen, O and Eliasson, R. (1987). Evaluation of commercially available kit for the colorimetric determination of zinc in human seminal plasma. International Journal of Andrology. 10(2):435 – 440

Kennedy, E., Mannar, V. and Iyengar, V. (2003). Alleviating hidden hunger: Approaches that work. International Atomic Energy Agency IAEA Bulletin, 45:1 - 6.

Lorke, D. (1983). A new approach to practical acute toxicity testing. Archives toxicology, 53:275 – 289.

Mohammed, M. S and EL-Nahal, D. M (2013). Nutritional evaluation of weaning food blends fortified with some carotenoids-rich vegetables. Journal of Applied Science Research, 9(3): 1573 - 1582.

National Advisory Committee on Micronutrient Deficiency (NACMD) (2003). Paper Distributed / Presented at the meeting of the NACMD, Abuja. March, 27th - 28th, 2003.

Nigerian Nutrition Network (NNN) (2000).Communiqués and Papers presented/distributed at the 1st Annual Nigeria Nutrition Network (NNN) meeting, Abeokuta. December, 12th - 13th, 2000.

Nnam, N. M. (2001). Comparison of the protein nutritional value of food blends based on sorghum, Bambara groundnut and sweet potatoes. International Journal Food Science Nutrition, 52: 25 - 29.

Nwala, C. O., Akaninwor, J. O. and Monanu, M. O. (2013). Nutritional studies of rats fed diets formulated from treated and raw samples of Jatropha curcas seed. International journal of Engineering Science Invention, 2(11): 14 - 19.

Odumodu, C. U. (2010). Nutrients and anti-nutrients content of dehulled soybean. Continental Journal of Food Science and Technology, 4: 38 - 45.

Okoh, P. N. (1998). Cereal grains In: Nutritional quality of plant foods: Osagie, A. U. and Eka, O. U. (Eds.) Post-harvest Research unit. University of Benin, Benin City. pp. 32 - 52.

Owolabi, A. O., Mac-Inegite, J. O., Olowoniyan, F. O. and Chindo, H. O (1996). A Comparative study of the nutritional status of children in villages in northern Nigeria using and not using soya beans. Food Nutrition Bulletin, 17: 42 - 48.

Oyaizu, M. (1986). Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Japanese Journal of Nutrition, 44: 307 – 315.

Prieto, P., Pineda, M. and Agular, M. (1999). Spectrophometric quantification of antioxidant capacity through the formation of a phosphomolybeduem complex: specific application to the determination of vitamin E. Analytical Biochemistry. 269:337 – 341.

Ramsey, W. N. M. (1952). The determination of iron in blood plasma or serum. Biochemical Journal 53: 227 – 231.

Sarkar, B.C., Chauhan, U. P. S. (1967). A new method for determining micro quantities of calcium in biological materials. Analytical Biochemistry, 20:155 – 166.

Solomon, M. (2005). Nutritive value of three potential complementary foods based on cereals and legumes. African Journal of Food and Nutritional Sciences, 5(2): 1 - 14.

Umar, I.A., Saidu, Y., Lawal, M. and Maigandi, S.A. (2010). Biochemical and Haematological Indices of weanly Albino Rats Fed Millet and Maize Based Complementary Weaning Food. Nigerian Journal of Basic and Applied Science, 18,1, 44-49

WHO (2003). Feeding and nutrition of infant and young children. Guidelines of the WHO European region with emphasis on the former Soviet Union. WHO Regional Publication European series. 87, 1 - 296

WHO (2008). Training course on child growth assessment. World Health Organization, Geneva.

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